Malvern Panalytical Zetasizer Advance series for particle size measurement of fluorescent samples

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The Zetasizer range includes some of the most widely used dynamic light scattering (DLS) instruments measuring particle and molecular size, particle charge and particle concentration from less than a nanometre to several microns of colloids, nanoparticles, polymers and proteins. The latest Zetasizer systems offer updated measurement features, hardware capabilities and software intelligence.

Non-invasive back scatter (NIBS) provides the versatility and sensitivity to measure over a wide concentration range, while multi-angle dynamic light scattering (MADLS) technology permits a high-resolution view into a sample’s size distribution for the measurement of particle and molecular size. The measurement of particle concentration is suitable for a wide range of materials, requires no or little dilution and is quick to use — all of which make it useful as a screening technique. This capability of the Zetasizer Ultra can even be applied to samples such as viruses and virus-like particles (VLPs), which were previously very challenging to measure.

A fluorescence filter wheel allows for NIBS size measurement of fluorescent samples like quantum dots, which can cause large background noise in the data. The fluorescence filter eliminates that noise and makes a measurement possible despite the presence of fluorescence.

Adding a polarising filter can clean up the optical signal by removing any depolarised light, which can be a source of noise in measurements caused by multiple scattering. This feature provides the versatility to measure over a wide concentration range, improving signal-to-noise without impairing overall system sensitivity. In addition to a vertical polariser, the Zetasizer Pro and Ultra have a horizontal polariser which measures depolarised light. A depolarised DLS signal can be used to detect rotational diffusion, which indicates differences in particle shape and whether particles are spherical or have surface differences.

For more information: https://www.atascientific.com.au/products/zetasizer-ultra/.