Azure Biosystems Sapphire FL Biomolecular Imager with resolution down to 5 μm

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The latest Sapphire FL Biomolecular Imager from Azure Biosystems is a multimodal scanner with image resolution now down to just 5 μm.

With customisable and user-changeable laser and filter modules, the product easily supports a broad range of wavelengths from 375 through to 900 nm — allowing UV, phosphor, visible and NIR imaging — and offers an optional chemiluminescent imaging module. A wide depth of field (25 x 25 cm) allows users to image western blots, six 96-well plates, 2D gels, protein and tissue arrays, tissue slides, living small animals, plants, phosphor screens, organ and tumour imaging, and much more.

High sensitivity allows femtogram detection of proteins labelled with common fluorescent dyes. Extended dynamic range EDR, when selected, allows imaging of both bright and weak bands without experiencing saturation. It extends to 24 bits of data, which is useful for samples that feature strong and weak expressing proteins.

The Sapphire FL imager makes imaging of live animals possible. When combined with appropriate tubing and nose cones, living mice can be fluorescently imaged under anaesthesia using one of five built-in anaesthesia ports. The wide imaging platform can accommodate specimens with depth (up to 4 cm), allowing mice, plants, multi-well plates, petri dishes, microscope slides and other large biological samples to be imaged.

The imager offers a 7 mm software-controlled adjustable focal plane, so users can scan their samples at multiple depths to see all their data has to offer. It scans between 5 and 1000 µm resolution for clear visualisation of detailed samples. The chemiluminescence module adds high-resolution, quantitative chemiluminescence and visible imaging.