Tomocube HT-2 live cell holotomography 3D fluorescence imaging microscope

Click2Contact

The HT-2 from Tomocube combines both holotomography and 3D fluorescence imaging into one microscope. The HT-2 facilitates three-dimensional fluorescence and optical diffraction tomography of live cells using structured illumination with minimal stress on cells. This technology is said to open the door to a variety of previously unexplored applications in time lapse imaging and 3D cell imaging in the fields of bioscience and life science, helping researchers and clinicians better understand, diagnose and treat disease.

The HT-2 is able to achieve label-free imaging of cells and allows users to conduct long-term tracking of specific areas within live cells. It has the capacity to deliver correlative analysis in 2D, 3D and 4D with HT and fluorescence images, and incorporates a customisable three-channel LED light source (385, 470 and 570 nm). HT-2 generates Z-stack images with a motorised Z-drive (step resolution: 150 nm).

TomoStudio, the HT series operating software, controls the system and visualises the captured image in a multitude of different ways. This software enables fast image acquisition (holotomographic images captured at 2.5 fps), holographic staining and in-depth data analysis.

The product offers correlative microscopy in one instrument, providing high-quality 3D images of both holotomography and 3D fluorescence for each sample. Quantitative data is marked with fluorescence and provides morphological (volume, surface area, projection area, sphericity and ellipticity), chemical (dry mass and concentration) and mechanical (cell deformability) properties of cells with a 3D refractive index (RI) tomogram. Moreover, the fluorescence image provides information about molecular specificity.

Live cell molecular and holographic imaging is produced with minimal stress on cells. Simultaneous measurement capability of time-lapse 3D RI tomography and fluorescence imaging allows long-time tracking of specific targets in live cells. The fluorescence image provides the position of specific target organelles or structures in live cells, and consecutive measurements of time-lapse 3D RI tomography enables the monitoring of cells and their structures with minimal stress.

For more information: http://scitech.com.au/microscopy-systems/3d-holographic-microscopy/.