Daicel’s Chiralpak and Chiralcel HPLC columns

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Daicel’s Chiralpak and Chiralcel HPLC columns use chiral stationary phases (CSPs) to separate racemates. The chiral HPLC columns are available not only for analytical use but also for commercial separations, offering good resolution of racemates, rapid and easy method development, durability and long service life. Daicel chiral columns, which are available in immobilised, normal and reverse phase types, are essential tools for the global pharmaceutical industry.

Daicel and Scientex have announced the Chiralpak immobilised type chiral HPLC columns, an addition to the I-series immobilised polysaccharide-based columns. Chiral HPLC methods are now widely used to determine optical purity of new pharmaceutical drug candidates; and since 2010, a growing proportion of this work has been undertaken by ‘immobilised’ stationary phases. The immobilised stationary phase is compatible with a wide range of organic mobile phases, which delivers higher sample solubility, and due to their outstanding solvent tolerance, high-efficiency separation of enantiomers. These columns are also more robust and ‘forgiving’ if the wrong solvent has been used for an assay.

Daicel’s I-series immobilised polysaccharide-based columns comprise the existing IA, IB, IC types and the new ID, IE and IF variants. Although immobilised columns have the stationary phase chemically bonded to a silica gel substrate, they behave like conventionally coated columns. By immobilising the CSP they are not only suitable for a broad range of applications, are highly selective, can take high sample loading and are very stable, but also have the ability to be used with many different organic solvents. Conventional coated polysaccharide columns suffer from a number of drawbacks including being limited in the selection of mobile phases that can be employed, inherent fragility (choose the wrong type of solvent and the column is destroyed!), poor baseline separation and unsuitability for many preparative applications. As a result of many customer requests, Daicel developed its range of immobilised columns to overcome these limitations. Solvents that can be employed on Daicel immobilised chiral columns that would normally destroy a coated CSP include toluene, chloroform, dichloromethane, acetone and DMSO.

The Chiralpak IA, IB, IC, ID, IE and IF immobilised columns feature: high selectivity and wide application range; compatibility with any organic solvent; ability to use the most appropriate solvent for the sample (important for preparative applications); ability to cope with a wider variety of conditions, thereby allowing a better chance of achieving baseline separations.