Molecule-thick coating to improve disease and drug testing

An international research team, led by the Lancaster University, has created a coating only one molecule thick that modifies the surface of sensor electrodes — with the results described in the journal Advanced Functional Materials.

The molecule in question, beta-cyclodextrin, has a funnel shape with a hydrophobic inside and a hydrophilic outside. The combination of these two properties works together to ensure that only molecules of the correct size and type will fit inside, thus ensuring the sensor only detects substances it is tuned for.

The process, which involves electrochemically grafting the beta-cyclodextrin onto the surface of graphite electrodes, is also easy to apply. It can be added in one simple step within minutes, without any special equipment or harmful chemicals — something the researchers believe makes the process cheap and scalable.

Although the use of cyclodextrin on sensors is not new, other methods to create similar sensor coatings have been, up until now, more complex and expensive. The new method is also claimed to produce sensors around 10 times more sensitive than the best existing sensors based on cyclodextrin, meaning it has the potential to detect specific molecules at much lower concentrations.

“This one-step modification holds substantial promise for the routine production of inexpensive yet robust and high-performing electrochemical sensors,” said Lancaster University’s Dr Stijn Mertens, lead researcher on the study.

“It is very selective, only picking up molecules that fit within the cyclodextrin. This is important because it means we can analyse samples without time-consuming and therefore expensive pre-treatment, such as separation from other substances. We believe that the excellent performance of the sensor may be related to its simple and well-defined structure, as the cyclodextrin is attached directly and strongly to the electrode surface.”

The researchers tested the sensor on dopamine — a neurotransmitter chemical that helps to send signals between nerve cells and is therefore very important in biochemistry. Dr Mertens said the researchers expect the sensor will work for many other small biomolecules or drugs, which “could prove useful in helping to diagnose diseases, such as Parkinson’s, or detecting drugs in athletes”.

The research is currently at an early proof-of-concept stage, though sensors with these coatings have the potential to be used by scientists within labs to test blood or tissue samples.

Image caption: A computerised model of beta-cyclodextrin. Image credit: Dr Abbie Trewin, Lancaster University.

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