Hawk Biosystems Violet 3.0 FLIM/FRET microscopy platform

Violet 3.0, by Hawk Biosystems, is an all-in-one microscopy platform for QF-Pro technology, bringing fluorescence lifetime imaging microscopy (FLIM) and Förster resonance energy transfer (FRET) to any lab. For those studying biomolecular interactions at the functional level, it enables deep insights into complex biological pathways. From enhancing immuno-oncology research to accelerating discoveries in cellular therapies, QF-Pro helps scientists to push the boundaries of biomedical innovation.

The comprehensive benchtop solution features a high-end modulated laser, sensitive image sensor and multi-channel LED for immunofluorescence. It includes two stage inserts allowing for the analysis of microwell plates or up to four standard microscope slides at a time. It is equipped with 10x, 20x and 40x dry objectives. It also includes four LED channels — 365, 488, 585 and 635 nm — for the illumination of secondary biomarkers of interest.

One desktop licence of QF-Pro software is included, allowing users of all expertise to easily run the device and generate groundbreaking data. Users can load their samples and create maps of regions of interest (ROIs); the platform then sequentially acquires all mapped ROIs and calculates a QF-Pro score and image per region. Post-acquisition analysis, such as the application of a threshold or change in Lookup Table (LUT), is automated, with the option of fine-tuning for more experienced users. All data can then be seamlessly exported into Microsoft Excel (data) or PowerPoint/PDF (images). The combination of Violet 3.0 and QF-Pro allows any laboratory or researcher to access the benefits of the software.

The microscopy platform’s ease of use supports both experienced scientists and beginners in exploring spatial biology with confidence.