Luxendo TruLive3D Imager for 3D multisample imaging of live specimens

The TruLive3D Imager from Luxendo is optimised for fast 3D multisample imaging of delicate live specimens in their native 3D environment. The optical concept, with dual-sided illumination and single-lens detection from below, enables fast acquisition speed, high-resolution imaging and minimal shadowing effects. The system is especially suitable for multiposition imaging of small embryos (eg, zebrafish, drosophila or mouse), 3D spheroids, organoids, oocytes, tracking stem cell development and differentiation, in vitro fertilisation research and monitoring, and more.

The sample chamber in the imager fits a large sample holder (length: 75 mm), which can accommodate tens to hundreds of samples. The ready-to-use TruLive3D Dishes are fully compatible with the TruLive3D Imager. The possibility to line up three disposable dishes extends the capacities of the system to enable the parallel imaging of samples grown under different media conditions.

The imager can achieve a resolution down to 255 nm in xy, enabling resolving subcellular structures in living samples free of phototoxic effects. Its compact, robust and vibration-free design provides stability even during long-term high-throughput experiments. Luxendo’s browser-based user interface offers a simple set-up and execution of multidimensional experiments, while real-time control is handled by an embedded controller to ensure microsecond precision timing independent of the PC’s performance fluctuations.

The system ensures easy sample mounting and handling, robust data acquisition pipeline and scaling of experiments. It enables minimised shadowing effects, 3D reconstruction, 5D/6D analysis, tracking of cellular and subcellular events, and morphological analysis.

Key features of the illumination optics include chromatic correction from 440 to 660 nm; light-sheet generation by beam scanning; flexible light-sheet thickness (2 to 6 µm); and two Nikon CFI Plan Fluor 10x W 0.3 NA water immersion objective lenses. Key features of the detection optics include one Nikon CFI Apo 25x W 1.1 NA water immersion objective lens; two spectral detection channels, each equipped with a fast filter wheel (10 positions and 50 ms switching time between adjacent positions); filters adapted to the selected laser lines; two high-speed sCMOS cameras; and a maximum frame rate of >80 fps at full frame (2048 x 2048 pixels of 6.5 µm x 6.5 µm size) and up to 500 fps at subframe cropping and peak quantum efficiency (QE) of 82% @ 560 nm.