Olympus FluoView FVMPE-RS deep-imaging multiphoton system

The Olympus FluoView FVMPE-RS multiphoton microscope system enables high-precision, ultrafast scanning and stimulation, allowing researchers to see deep within specimens, take measurements at high speeds and capture images even under demanding conditions.

The product is suitable for any field that requires precise colocalisation, uncaging, simultaneous imaging/stimulation, real-time signal processing or multipoint mapping. Its design offers adaptability for researchers who design their own custom-built systems.

The precision timing allows for microsecond repeatability and control of multiple imaging and stimulation protocols, as well as millisecond repeatability over days of time-lapse imaging. Complex multiposition imaging or optogenetic stimulation protocols can be accomplished using the stage control and sequence manager.

The system captures 438 fps at 512 x 32. It captures full-frame, 512 x 512 images at 30 fps without any reduction of the field of view. Its scanner unit combines a resonance scanner with a galvanometer scanner to provide both speed and definition. An optional third galvanometer scanner is also available. The multipoint mapping capability allows ultrahigh-speed measurement of rapid fluctuations in groups of cells.

The product provides multicolour, multiphoton excitation and imaging with a choice of lasers, along with a four-axis auto-alignment capability for precise colocalisation and coalignment without pixel shift. With its multiple-laser-line performance, researchers can work with two IR laser lines simultaneously while stimulating the sample with visible light.

The system offers high-efficiency multiphoton excitation at wavelengths of 1300 nm, with the ability to support wavelengths of up to 1600 nm. Objectives that offer working distances of 2, 4 or 8 mm deliver super-deep imaging. Light collection efficiency is improved by the system’s optimised light path. In Deep Focus Mode, researchers can tune lasers for deep-tissue imaging to achieve signal improvements of 20% or more.

The system’s multichannel analog box for triggering, synchronisation and signal input provides support for advanced electrophysiology studies and other applications requiring external device triggering. Enhanced temperature stability and a rigid frame make the product suitable for live cell experiments. It will be sold as part of the line of Olympus imaging microscope systems.