Refeyn MassFluidix HC upgraded microfluidic system

Refeyn has introduced a next-generation microfluidic chip and other improvements to its MassFluidix HC microfluidic system, in order to enhance the rapid dilution of highly concentrated samples and improve insight into low-affinity and transient biomolecular interactions. The updates are designed to save users time while enabling reproducibility across experiments and labs and supporting a broader range of applications.

A key addition to the system is a second-generation microfluidic chip which enables mass photometry measurements in five channels and features even faster sample dilution — in less than 37 ms. Usage is simplified with a channel redesign that removes the need for a bubble trap and introduces luer connectors that enable easy connection of tubing to the chip. The ready-to-use chip is individually packaged, allowing users to open just one at a time as needed.

Further improving ease of use, software integration now means that the rapid dilution system can be readily operated directly via Refeyn’s Acquire^MP mass photometer control software. An automatic microfluidic chip channel detection feature within this software also simplifies workflows by guiding correct positioning of the chip for each measurement.

The upgraded system’s rapid dilution allows the characterisation of samples at high concentration. This is particularly useful as a quality check for techniques that require higher concentrations, such as cryoEM and crystallography, as well as for detecting and determining stoichiometry for weak affinity protein-protein interactions. This has implications for traditionally difficult-to-characterise sample types, such as PROTACs and multi-component complexes; in addition to more common applications, such as antibody-receptor binding, and as an orthogonal technique to other binding characterisation techniques.