Thermo Scientific Vanquish UHPLC system

The Thermo Scientific Vanquish UHPLC looks different from standard UHPLC systems. The monolithic case contains an instrument that combines the ruggedness of an integrated system with the flexibility and serviceability of a modular system. The product stands about 25% lower than comparable modular stacks for safety and convenience.

Thermo Scientific Accucore Vanquish UHPLC columns are designed to optimise performance. The columns feature 1.5 µm solid core particles utilising Core Enhanced Technology to take advantage of the system’s 1500 bar maximum pump pressure and flow rate up to 5 mL/min for ultrashort diffusion path lengths and highly efficient separations.

SmartFlow pumping technology is designed for reproducible retention times and low baseline noise to enhance detection sensitivity. Temperature control capabilities, including direct heating and forced air temperature control modules, enhance separation efficiency and selectivity as well as retention time reproducibility. An insulated autosampler compartment with air-to-air cooling protects vials from water condensation. The hard carbon-coated ceramic injection valve and pump pistons are designed to deliver consistent, reproducible performance for 200,000 injections.

The gold-standard Thermo Scientific Dionex Chromeleon Chromatography Data System can calibrate the system automatically and unattended. eWorkflow and Intelligent Run Control automation are designed to enhance data quality and instrument efficiency. Instant data reprocessing paired with Cobra Peak Detection and Smart Link can speed up data review significantly compared to manual procedures. Chromeleon software supports chromatography and mass spectrometry functions simultaneously.

The product’s Diode Array Detector is designed to generate data over a wide linear range with high signal-to-noise performance. Low-dispersion 10 and 60 mm flow cells provide high sensitivity levels and wide linearity. Fused-silica LightPipe flow cells are designed to exploit low baseline drift for reproducible quantitation. Users can acquire up to 10 signal channels simultaneously and a spectrum 3D field at 200 Hz. Sensitivity, linearity and spectral resolution can be tailored to suit the application using four programmable optical slit widths from 1 to 8 nm.