Waters Intact Mass app, ACQUITY Premier and XBridge Premier Protein BEH C4 300 Å columns

The Waters Intact Mass app on waters_connect allows scientists using the BioAccord LC-MS System to confirm the mass of biomolecules and impurities made by synthetic or recombinant processes nearly twice as fast as other commercially available options, the company claims. The app is available for new BioAccord LC-MS Systems and as an upgrade to previously installed systems.

Intact mass analysis is routinely performed during all stages of the development of biological drugs including proteins, peptides, oligonucleotide therapies and conjugates. In early stages of drug discovery, biochemists must analyse hundreds or even thousands of different samples per week. To help speed up this process, the app provides a fast and automated solution to facilitate mass confirmation and purity determination of novel biotherapeutics. The application features intelligent automated deconvolution to process sample results within minutes of their capture, with minimal user input.

Complementing the app is a line of analytical columns for analysing intact biomolecules and their subunits. The ACQUITY Premier and XBridge Premier Protein BEH C4 300 Å columns for the BioAccord LC-MS System feature MaxPeak High Performance Surfaces (HPS) technology that prevents the loss of sample analytes due to adsorption of phosphorylated and carboxylated molecules between the sample and metal surfaces of both the LC system and column. This enables high sensitivity for low-level intact mass analysis and for intact mass analysis of phosphorylated proteins and low-level subunits of monoclonal antibodies.