Enabling new levels of quantification

Using the SCIEX Triple Quad™ 7500 LC-MS/MS System – QTRAP^® Ready, powered by SCIEX OS Software

The ability to achieve high levels of sensitivity while retaining quantitative performance is key to achieving robust analytical results.

The growing number of demanding workflows requiring reliable quantification of more analytes per assay, in increasingly complex matrices, highlights the need for a sensitive MS instrument capable of providing the high level of performance that can meet these analytical challenges while providing the highest levels of robustness. The SCIEX Triple Quad™ 7500 LC-MS/MS System – QTRAP^® Ready is enabling new levels of quantification across a large suite of sample types and workflows. The greater sensitivity is achieved through the introduction of hardware features that enable significant gains in the generation, capture and transmission of ions including the OptiFlow^® Pro Ion Source (1 µL/min to 3 mL/min), D Jet™ Ion Guide and E Lens™ Technology.^1,2,3

Figure 1: Sensitivity gains for SCIEX 7500 System over QTRAP 6500+ System. Large numbers of analytes were run in various matrices on both systems and the peak areas and S/N gains were measured. (Left) Example data from propranolol in rat plasma, area gain of 9x with S/N gain of 3x. (Right) Summary of comparison of 1244 MRMs in positive and negative mode across 10 studies (pesticides, drugs, peptides), average peak area gain across the compounds was 7x.

Figure 2: High robustness observed across 2700 injections. To test robustness, many injections were performed using a very complex matrix (black tea dosed with carbendazim) using a short 4 min LC gradient at 400 µL/min. The raw peak area of carbendazim was then plotted vs. injection number and the variance across these peak areas was computed to be 4.05% for 2700 injections.

Key innovations in the SCIEX Triple Quad 7500 System – QTRAP Ready

• D Jet Ion Guide — improved ion capture and transmission, combined with an orifice with increased sampling area for increased ion sampling, resulting in greater sensitivity
• E Lens Technology — improved ion generation through more energetic ESI droplet desolvation and more efficient ion collection
• OptiFlow Pro Ion Source — designed for robustness and ruggedness with modular architecture to future proof the lab
• QTRAP ready⁴ — triple quadrupole functionality for quantification, plus full scan linear ion trap MS/MS for confirmation and MRM³ for selectivity⁵
• Detection system with high energy dynode and pulse counting detector — fast polarity switching (5 msec) and up to 6 orders of magnitude across the linear dynamic range
• SCIEX OS Software — designed for intuitive and streamlined data acquisition and data processing, all on one single platform⁶

Figure 3: D Jet Ion Guide – improved ion capture and transmission, combined with an orifice with increased sampling area for increased ion sampling, resulting in greater sensitivity.

References

1. Sensitivity gains for the evolution of routine bioanalysis. RUO-MKT-02-11885-A.
2. Highly sensitive pesticide analysis in baby food. RUO-MKT-02-11958-A.
3. High Sensitivity Peptide Quantification Workflow. RUO-MKT-02-11882-A.
4. Powerful scan modes of QTRAP^® system technology. RUO-MKT-02-8611-A.
5. MRM³ quantitation for highest selectivity in complex matrices. RUO-MKT-02-2739-A.
6. SCIEX OS Software: Transform your samples into meaningful analytical answers

For more information: https://sciex.li/SCIEX7500.

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_{Related to RUO-MKT-02-11886-A.}

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