Kuhner Feeding Technology

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Kuhner Shaker is known for high-quality shaking incubators and now manufactures an innovative and easy-to-use solution for screening under fed-batch conditions in microtitre plates, shake flasks and spin tubes.

While screening for more efficient and productive strains, the user is frequently confronted with one challenge: a large part of process and strain development is carried out under batch conditions. In this operating mode, an excess of nutrient is available at the beginning of the cultivation, whereas the final production process is usually carried out under fed-batch conditions.

Kuhner’s polymer-based feeding tool enables cultivations in a reproducible fed-batch mode during primary screening. As screening conditions will be similar to conditions in the production process, any statement about the productivity of a clone should be more accurate. Negative effects of batch conditions, eg, overflow metabolism, oxygen limitation and substrate inhibition, can be avoided.

Feeding Technology can be used to cultivate a wide spectrum of organisms: from typical model organisms such as E. coli and S. cerevisiae, to P. pastoris and H. polymorpha. The feeding tools are integrated easily into existing screening processes and can be used with standard laboratory equipment.

Kuhner Feeding Technology offers flexibility regarding possible substrates, release rates and culture vessels. The technology is ready to use and delivered sterile.