Proteintech FcZero recombinant antibodies for flow cytometry

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Proteintech has designed recombinant antibodies featuring the company’s FcZero backbone, engineered to silence the Fc domain for clean flow cytometry data.

Non-specific binding of antibodies to Fc receptors in a sample can cause background signal in data. The FcZero-rAb backbone features a mutation in the Fc receptor binding region to eliminate Fc receptor binding. The resulting data is cleaner than using a conventional antibody and Fc receptor block, Proteintech says.

The FcZero recombinant antibody backbone features AccuBright site-specific conjugation technology, designed to allow precise control of the location of the fluorophores on each IgG backbone. This means that fluorophores do not block the antigen-binding region of the antibody. AccuBright also allows for good control over the degree of labelling (DOL), or number of fluorophores conjugated to each antibody, so that signal intensity is consistent from lot to lot.

Popular gold standard clones for flow cytometry are now available with an FcZero-rAb backbone. The antigen-binding regions of these classic clones are combined with a Mouse IgG2a backbone that includes the FcZero mutation to the Fc receptor binding region and AccuBright site-specific conjugation. These antibodies have well-established specificity, plus the high purity and zero non-specific background from the Fc receptor binding.

FcZero-rAb has all the advantages of Proteintech’s Uni-rAb recombinant antibodies, including good specificity, high affinity, lot-to-lot consistency and high purity. It is available in carrier-free formulations.

For further information, contact United Bioresearch Products, the distributor of the full range of Proteintech products in Australia, or visit https://www.ptglab.com/products/flow-cytometry-antibodies/recombinant-antibodies-for-flow-cytometry/fc-silenced-antibodies/.