Enzo Life Sciences DiSH kit for detection and production of antibodies

Click2Contact

Improving the product development cycle for new therapies is a constant challenge for drug discovery researchers. With the DiSH kit, Enzo Life Sciences provides a tool to enable rapid detection and production of highly specific monoclonal antibodies.

The Direct Selection of Hybridomas (DiSH) kit is a system in which mAb-secreting hybridoma cells co-express significant amounts of the membrane form of the secreted immunoglobulin (Ig) on their surfaces. Subsequently, hybridomas with high affinity for the target antigen are labelled.

Once labelled, they can be isolated from among a pool of hundreds of thousands of cells in a matter of hours using fluorescence activated cell sorting (FACS) or magnetic separation. The total elapsed time from harvest to the first screening is typically 16 days, resulting in improved efficiency for detection of highly specific mAb.

The kit contains an Sp2ab myeloma fusion partner from Abeome, a genetically modified cell line which results in hybridomas that both secrete antigen-specific antibody and express antibody on the cell surface. Surface-bound antibodies allow antigen-specific hybridomas to be labelled and efficiently sorted by fluorescence-activated cell sorting (FACS) or magnetic separation.

Culture, fusion, selection and recovery media for creation and isolation of hybridomas are also included in the kit.