ProteinTech Western blot loading control antibodies

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Western blotting is an application that requires endogenous or internal controls such as proteins and peptides, as they are widely used for determining specific protein levels via Western blot quantification; internal control proteins are detected for Western blot loading controls following primary detection of a protein of interest. This step is used to standardise results and normalise for any errors that creep into a Western blot experiment, such as sample loss through loading at SDS-PAGE or Western blot transfer.

The loading control candidates for Western blotting are usually proteins with high and constitutive expression. The most basic criterion for a loading control is that its levels remain unchanged throughout an experiment, regardless of tissues or cell types used and how they are handled. This means control candidates require careful selection; even bastions of the loading control repertoire - such as β-actin and α-tubulin - can be affected by the conditions of an experiment.

ProteinTech produces antibodies from whole protein antigens for use as Western blot loading controls. The range includes: Actin - the most abundant proteins in the typical eukaryotic cell, accounting for about 15% of total protein in some cell types; COX-4 - expressed at a dependably high level; GAPDH - high and constant expression in most tissue and cell types; and VDCA1 - ubiquitously expressed in tissue and highly conserved throughout multiple species.