Advanced Analytical Technology High Sensitivity Fragment Analysis Kits for cfDNA quality control

Sequencing cell-free DNA (cfDNA) fragments, a biomarker used to detect and treat cancer via a liquid biopsy needs an optimal DNA concentration during flow-cell loading. Advanced Analytical Technology’s line of High Sensitivity Fragment Analysis Kits for its Fragment Analyzer Automated Capillary Electrophoresis System can measure the size and concentration of the three common cfDNA fragments — the ~165 bp mono-nucleosome, the ~350 bp di-nucleosome and the ~565 bp tri-nucleosome — using only 2 mL of sample.

The kits use internal standards, which enables DNA quantification within 25%. The sizing accuracy for the small fragment and NGS kits is within 5%, while the accuracy of the large fragment kit is around 15%. Runs are completed in less than an hour.

The kits contain everything required for an optimised capillary electrophoresis experiment on cfDNA.