Bio-Rad Laboratories StarBright Red 715, 775 and 815 Dyes and expanded antibody markers

Bio-Rad Laboratories has launched three more StarBright Red Dyes — StarBright Red 715, 775 and 815. Now totalling 32 dyes spanning multiple lasers, the range expands translational and immunological research capabilities by providing greater choice and flexibility in conventional and full-spectrum multicolour flow cytometry panels.

The StarBright Dyes — proprietary fluorescent nanoparticles conjugated to flow-validated antibodies against key immunological targets — are designed to offer researchers high brightness with narrow excitation and emission profiles for precise resolution. Joining StarBright Red 670, StarBright Red 715, 775 and 815 are excitable by the 640 nm laser and are understood to be brighter than or as bright as other current 640 nm excitable comparison dyes that emit at a similar wavelength. With minimal background compared with tandems containing cyanine dyes, the dyes are also stable and can be fixed in formalin or alcohol-based fixatives.

The dyes are compatible with most experimental protocols and flow cytometers, including the Bio-Rad ZE5 Cell Analyzer, without the need for special buffers. Minimal lot-to-lot variation helps to ensure reproducible and consistent staining, and the dyes are resistant to photobleaching with no loss of signal in fixation.

In addition, the company has launched 29 additional highly validated antibodies conjugated to its StarBright Violet 570, 515, 440, 670, 790 and 475 Dyes. Expanding the range of human and mouse targets and introducing dog, cow and pig targets, the additional 29 antibody markers will soon be available on all StarBright Dyes.