Biosynth Microvesicle Extraction (ME) Kits

Biosynth’s range of Microvesicle Extraction (ME) Kits, for exosome extraction, allows separation of extracellular vesicles (EVs) from complex mixtures. By using the highly specific Heat Shock Protein (HSP) binding Vn96 peptide, EVs can be removed from urine or plasma as well as cell culture media.

Isolating EVs from plasma samples has always presented challenges compared to doing the same with urine or media samples. Biosynth has experimented with and refined a protocol to optimise this for the Vn96 platform to enrich EVs in under an hour.

Two different versions of the kit are available: one for urine or media samples, and one for plasma. Each kit uses the same Vn96 reagent, but the recommended protocols are slightly different. The company has also developed a sample dilution buffer for plasma samples that should improve clarity of results.

Biosynth has specialised methods to include helpful tips and protocols for several types of analyses or studies, most notably a method for releasing the EVs from their bound state with the Vn96 peptide, thereby making them available for subsequent functional studies.