Fluorescent peptide targets

Organelle and Cellular Lights reagents are recombinant baculoviruses that, on entry into mammalian cells, direct the expression of fluorescent proteins (cyan, green, yellow, orange and red) fused to peptide targeting sequences. The targeting sequences allow accurate and specific staining of subcellular structures and organelles, as well as proteins involved in important cellular processes. These reagents are easy to use for colocalisation studies in live cell imaging applications where high spatial and temporal resolution is required by scientists interested in cellular pathways and development.

As the reagents are based on fluorescent proteins, they are also fixable and therefore suitable for multiplex applications using antibodies — a feature particularly attractive for HCS applications.

There is no need for researchers to clone their own constructs, grow bacteria, purify plasmids and make transfection complexes for each experiment. Organelle and Cellular Lights reagents are well characterised, safe and ready-to-use fluorescent protein intracellular landmarks.

The reagents provide efficient, non-cytopathic delivery to multiple cell types (including primary and stem cells) — pre-packaged in BacMam format. Currently more than 70 cell types have been shown to be amenable to BacMam transduction (hematopoietic lines are an exception).

Every batch of Organelle Lights is also pre-packaged at a constant concentration, so users can expect reliable, reproducible results.