Shimadzu high-performance liquid chromatography application note

Shimadzu has published an application note to illustrate the capabilities of UHPLC and Triple Quadrupole LC/MSMS [LCMS-8030] for quantitation and identification of total aflatoxins in food.

Aflatoxins are mycotoxins that are extremely carcinogenic and acutely toxic. In Japan, foods in which the total aflatoxins (sum of B1, B2, G1 and G2 aflatoxins) are detected at levels exceeding 10 μg/kg are in violation of the Food Sanitation Act1).

Previously, Shimadzu introduced examples of simultaneous analysis of these four substances (B1, B2, G1 and G2) using HPLC and UHPLC. In accordance with the test method specified in the notification of 16 August, 2011, after pretreatment using an immunoaffinity column, Shimadzu conducted (1) quantitation using an HPLC system compliant with the test method, (2) identification using a LC/MS system compliant with the test method, and (3) rapid quantitation and identification using the Nexera UHPLC.

The application note presents these analyses in addition to the results of the validation.

The application note is available for download at: http://www2.shimadzu.com/applications/LC/L435.pdf.