Agilent NovoCyte Opteon Spectral Flow Cytometer

Click2Contact

Flow cytometry is a powerful single-cell detection technique used in all aspects of scientific research to probe cell phenotype and function.

In traditional flow cytometry, dedicated bandpass filters are used to capture the maximum fluorescence emission. Although the design of multiparameter panels has had some success using this approach, limitations exist due to the size of the panel and use of certain dye/fluorophore combinations and resolution.

The advent of spectral flow cytometry has led to advancements in supporting more markers, greater flexibility and better data resolution. The Agilent NovoCyte Opteon is a cutting-edge spectral flow cytometer designed to revolutionise cell analysis research.

The NovoCyte Opteon Spectral Flow Cytometer is equipped with up to five lasers and 73 detectors. A dual 488 nm-SSC (B-SSC) and 405 nm-SSC (V-SSC) allows detection of particles as small as 80 nm without adjusting the settings to detect larger cells within the same sample. Furthermore, with this dual SSC, whole blood analysis can be separated into platelets, red blood cells and white blood cells without the need for any lysis protocols.

Real-time monitoring of instrument status enables uninterrupted data acquisition at varied high and low sampling rates. The intuitive Agilent NovoExpress software features flexible reference control set-ups and autofluorescence subtraction capability. This is all augmented by walkaway automation with the NovoSampler S autosampler, which is flexible (runs various plate and tube rack formats) and lab automation friendly.

The Agilent NovoCyte Opteon Spectral Flow Cytometer is designed to enable high-dimensional multiparametric cell analysis.