Mabtech IRIS 2 FluoroSpot and ELISpot Reader

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The Mabtech IRIS 2 is an advanced FluoroSpot and ELISpot reader designed to analyse up to four analytes simultaneously with accuracy and speed. The device features intuitive software for easy data management and self-calibration and can integrate into automated workflows with robotic arms for larger laboratory set-ups and projects.

The plug-and-play system is designed to streamline workflow. Simply load the plate, select the assay and press “read”. The user-friendly Mabtech Apex software ensures a smooth onboarding process, making spot analysis easy for users of all experience levels. Its intuitive layout and automatic data export accelerates the transition from data acquisition to analysis.

After reading, users can modify parameters like brightness, contrast, spot intensity and size thresholds without affecting the original data, leveraging uncompressed spot signals captured directly from the camera sensor. The Apex software supports a variety of file formats to simplify data management.

The new FociSpot assay application enables automated foci counting in a 96-well plate format. It detects foci using immunostaining with virus-specific mAbs and a precipitating substrate reaction like ELISpot. Enhanced by the patented RAWspot technology integrated into the software, it optimises spot centre detection for precise counting and identification of cells secreting multiple analytes in FluoroSpot. Furthermore, it introduces the concept of relative spot volume (RSV), extracting a three-dimensional volume for each spot to accurately reflect the amount of secreted analyte.

Mabtech’s Performance Qualification (PQ) plate verifies instrument quality and ensures reproducibility, allowing users to conduct quantitative and qualitative tests to identify any inconsistencies.