Malvern MicroCal PEAQ-ITC system and analysis software

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Isothermal titration calorimetry (ITC) is one of the most robust and convenient techniques to examine biomolecular interactions by detecting any reaction heat that takes place. The technique delivers direct, label-free measurements of binding affinity (K_D), stoichiometry (n), free energy (ΔG), enthalpy (ΔH) and entropy (ΔS) in a single experiment. However, the technology has not always been widely applied particularly in drug discovery because of the significant protein and compound consumption associated with conventional methodologies and the broad affinity range that needs to be measured.

The Malvern MicroCal PEAQ-ITC addresses this and many other challenges with a series of enhanced capabilities. Featuring a wide affinity range, fast response time and high signal to noise, the product enables the analysis of weak to high affinity binders (at high and low concentrations) with high reproducibility. The non-reactive Hastelloy cell ensures chemical resistance and compatibility with biological samples while the precision pipette enables smaller injection volumes.

Software delivers fast analysis with high data sensitivity and fully automated options for unattended operation, minimising user subjectivity. Moreover, the analysis software package allows for the determination of the active concentration of the target protein or the ligand concentration or both when the appropriate controls are used.

Additional tools simplify the fitting of complex binding isotherms that may be seen when titrating with mixtures of enantiomers, some competition experiments or with targets that have more than one binding site. These benefits highlight the value of the additional features integrated within the analysis software and the advantages the system provides for powerful data interpretation.

For more information: https://www.atascientific.com.au/products/malvern-microcal-peaq-itc/.