Malvern Panalytical Creoptix WAVEsystem for label-free analysis of binding kinetics and affinity

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Conventional bioassays like ELISA have relied on enzyme or fluorescent labels to detect and monitor biomolecular interactions; however, these labels can introduce artefacts, complicating data interpretation. Such assays are also incapable of providing kinetic insights, such as binding speed or duration. While surface plasmon resonance (SPR) offers label-free analysis, it often captures only a snapshot in time and is prone to issues like clogging and non-specific binding, which can compromise results.

Malvern Panalytical’s Creoptix WAVEsystem overcomes these limitations by delivering a robust, label-free technology for real-time analysis of biomolecular interactions. The system delivers deep insight into previously undetectable interactions and provides detailed kinetic rate parameters and affinity constants (k_a, k_d, K_D), as well as binding specificity, even for challenging samples and complex biological matrices.

The power behind the Creoptix WAVEsystem is in its patented Grating-Coupled Interferometry (GCI) technology, paired with the innovative, non-clogging WAVEchip microfluidics system. GCI measures the evanescent wave across the entire sensor surface, offering high sensitivity. The kinetics of weakly binding fragments and large molecules with high affinity and slow dissociation can be measured simply (even native proteins in complex matrices) without purification.

For applications requiring higher throughput, the system’s waveRAPID method enables faster screening by generating a pulsating concentration profile, streamlining workflows for screening studies. Additionally, calibration-free concentration analysis (CFCA) provides a quick approach for quantifying active protein concentration.

For more information: https://www.atascientific.com.au/products/creoptix-wave/.