Malvern Panalytical NanoSight Pro for rapid protein thermal stability analysis

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When developing safe and effective therapeutics using novel drug delivery systems, ensuring sample purity, stability and consistency is essential. Equally important is obtaining detailed insights into these complex systems to understand the intricate relationship between the composition of these systems and their therapeutic effects. A key aspect of this research is investigating protein aggregation, a process that can trigger an immune response (immunogenicity) and compromise therapeutic efficacy. Since thermal reactions are a key driver of protein aggregation, studying the thermal stability of proteins provides valuable insights into their lifespan, storage requirements and transportation conditions.

The Malvern Panalytical NanoSight Pro with Nanoparticle Tracking Analysis (NTA) technology offers an easy and effective way to investigate protein thermal stability. NanoSight can measure protein size and monitor its change (which indicates the beginning of denaturation and aggregation) while increasing the measurement temperature. Thermal ramp experiments can be scheduled in the NS Xplorer software to support protein aggregation studies with extended temperature ranges up to 70°C. NanoSight not only yields information of early-stage formed aggregates and their concentration, it also provides a visual assessment of the aggregation state. High-resolution size and concentration data are generated within minutes, enabling rapid evaluation of both sample stability and complexity.

NanoSight Pro has become a standard toolbox in the characterisation of biomaterials including extracellular vesicles, drug delivery systems, viral vectors and protein aggregates. Packed with smart features, the NanoSight Pro provides high sensitivity in biologics detection, high reproducibility and enhanced fluorescence measurements for detecting sample subpopulations including surface markers and internal cargo analysis. Powered by machine learning algorithms, measurement subjectivity is reduced and automated processing should result in easy, quick and accurate characterisation for all users.

For more information, visit https://www.atascientific.com.au/products/malvern-nanosight-pro/. To request a demo, contact ATA Scientific.