Malvern Viscosizer TD biomolecule measurement

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The Malvern Viscosizer TD is an automated characterisation tool that uses Taylor dispersion analysis (TDA) to enable ultralow-volume, solution-based molecular size and stability measurement capabilities, combined with relative viscosity assessment.

Measurements of hydrodynamic size in different solution conditions give insight into the conformational stability and self-association behaviour of a candidate drug. However, these measurements can be challenging due to the limited availability of target proteins, as well as interference from complex buffer and excipient backgrounds.

The Malvern Viscosizer TD addresses these issues by enabling label-free analysis of biomolecules in complex formulations, particularly at low concentrations and low volumes. The stability of small molecules, peptides, proteins and samples with mixtures can be monitored even in the presence of excipients and surfactants, without measurement interference.

UV detection allows measurements at low concentration down to microgram quantities. Measurements are not adversely affected by the presence of low levels of aggregates, so samples can be run without dilution or filtration. Using microcapillary flow methodology, the product also allows complementary relative viscosity measurement on the same sample.

Features include: temperature-controlled sample storage and measurement; nanolitre-scale sample volume for sizing; and microlitre-scale sample volume for relative viscosity. The capability to work in biologically relevant solutions makes the instrument complementary to established tools for protein characterisation such as dynamic light scattering (DLS) and size-exclusion chromatography (SEC).