WPI MICRO-ePUMP Microinjector

Click2Contact

Designed to simplify intracellular injection and a variety of other microinjection tasks, the MICRO-ePUMP from WPI uses carefully regulated air pressure for injecting cells with fluid. Injected volumes range from microlitres to nanolitres. The port supplies positive pressure for high-pressure ejection. The pressure port maintains a low positive ‘compensation’ pressure to the injecting pipette between injection pulses to prevent fluid uptake through capillary action.

The product is designed to inject very small quantities of fluids, such as drugs, into cells or small organelles. Pressure injection is an especially useful alternative to electroionophoresis, since it does not mandate the use of charged ions. The compensation pressure is a constant low pressure that eliminates any capillary action front-filling of the pipette and then the precise burst of regulated higher pressure is activated with the foot switch that is included.

WPI’s MICRO-ePORE Pinpoint Cell Penetrator technology is embedded inside the MICRO-ePUMP. When the researcher enables the MICRO-ePORE, it delivers a highly localised voltage signal to a targeted injection site to facilitate penetration with minimal trauma. The researcher determines the amplitude and frequency of the signal that best suits the application. The signal originates in the control box, and it is transmitted through the electrode interface cable to the microelectrode holder. A silver wire is used to transmit the signal into the electrically conductive substance being injected. A reference electrode is used to place the media at 0.0 V potential with reference to the generated voltage.