WPI MICRO-ePUMP, μPUMP and PV850 Microinjectors

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WPI’s new-generation pressure injectors are quickly replacing the legacy line of pneumatic valve PicoPump pressure injectors, the PV820 and PV830 having been a staple in the microinjection market for decades.

The company’s three latest injectors facilitate any type of microinjection that involves glass micropipettes. They are the MICRO-ePUMP Microinjector with an integrated cell penetrator and internal pressure source; the μPUMP Microinjector with internal pressure source; and the PV850 Microinjector for use with external pressure sources.

WPI Microinjectors use carefully regulated air pressure for injecting cells with fluid. Injected volumes range from picolitres to nanolitres. The port supplies positive pressure for high-pressure ejection. The pressure port maintains a low positive ‘compensation’ pressure to the injecting pipette between injection pulses to prevent fluid uptake through capillary action.

The WPI Microinjectors are designed to inject very small quantities of fluids, such as drugs, into cells or small organelles. Pressure injection is an especially useful alternative to electroionophoresis, since it does not mandate the use of charged ions. Two different positive pressures may be applied: one for ejection at high pressure and a second, lower pressure to prevent back filling of the pipette by capillary action.