Beckman Coulter Life Sciences CytoFLEX mosaic Spectral Detection Module

Wednesday, 19 March, 2025 | Supplied by: Beckman Coulter Australia


Beckman Coulter Life Sciences, an Operating Company of Danaher Corporation, has released a modular solution for the spectral flow cytometry industry: the CytoFLEX mosaic Spectral Detection Module.

When connected to the CytoFLEX LX or S Flow Cytometers, the module delivers high fluorescence sensitivity for dim and complex multicolour experiments and can detect nanoparticles as small as 80 nm. It provides two unmixing algorithms and up to 10 autofluorescence channels, with up to 88 detection channels. The rich data resulting from spectral analysis can help untangle the complexities of biological systems and diseases quickly, with a nuanced understanding of immune cell subsets, their functions and interactions, using simultaneously detecting multiple markers.

In contrast to conventional flow cytometry, spectral flow cytometry acquires the full emission spectrum of each antibody-associated fluorophore across all lasers, rather than measuring only the peak of emission. This is understood to deliver a more detailed and precise characterisation of cellular properties to advance understanding of complex biological systems and diseases within each sample versus legacy approaches.

The product is focused on pairing ease of use with powerful, sensitive acquisition technology to remove the intimidation encountered in many flow cytometry instruments. Investigators can easily switch between their true conventional detection system and the spectral detection system, while maintaining a familiar sample management and user experience, all without having to invest in a separate instrument.

The module is powered by an algorithm that is capable of improving resolution, along with unmixing accuracy checks for efficiency. CytExpert software for spectral reduces the need for extensive training and can speed up the transition to spectral technology. Additionally, the module comes with tools to ease the full spectral workflow, with access to the FluoroFinder panel builder and a Cytobank premium licence to provide all the tools researchers need to go from sample preparation to analysis of complex spectral data in an easy manner.

The module is available in two product configurations: CytoFLEX mosaic 88 for CytoFLEX LX Flow Cytometer and CytoFLEX mosaic 63 for 4-laser CytoFLEX S Flow Cytometer.

Online: www.beckman.com.au
Phone: 1800 060 878
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