Active Motif CUT&Tag-IT Assay Kit

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Cleavage Under Targets and Tagmentation (CUT&Tag) is a method to investigate genomic localisation of histone modifications, and some transcription factors, which reveals interactions between proteins and DNA or identifies DNA binding sites for proteins of interest.

Unlike MNase-Seq or ATAC-Seq methods which target open chromatin, and are therefore dependent on chromatin accessibility, CUT&Tag utilises an antibody-based enzyme tethering strategy to target specific histone modifications or proteins to reveal chromatin-binding information that is specific to those sites or proteins of interest.

CUT&Tag is based on the same principles as ChIP-Seq, but with several changes to the protocol that are advantageous in certain situations. Instead of the sonication of fixed chromatin and immunoprecipitation steps performed in ChIP-Seq protocols, in CUT&Tag, fresh (not frozen) unfixed cells are bound to concanavalin A beads and the antibody incubation is performed with cells in their native state. Directly following antibody binding, the chromatin is digested and NGS libraries are prepared in a single step by tagmentation using the protein A-Tn5 (pA-Tn5) transposome enzyme that has been pre-loaded with sequencing adapters.

CUT&Tag can rapidly produce high-quality results from less starting material than ChIP-Seq and enable robust analysis from lower sequencing depths. CUT&Tag-IT Assay Kits from Active Motif are compatible with as few as 5000 cells; exhibit low background signals, enabling low sequencing depth; and come complete with all reagents required along with an optimised protocol.

For more information: https://www.activemotif.com/catalog/1318/cut-tag-it-kit.