Azure Cielo real-time PCR system

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The Azure Cielo real-time PCR system from Azure Biosystems is a qPCR system designed to provide high-quality data through high-performance optical technology, broad-spectrum detection capability, high specificity, precision, fast run times and reproducible qPCR data. Applications include quantitative and qualitative gene expression analysis, miRNA analysis, genetic mapping, genetic fingerprinting, NGS library quantification, pathogen quantification and six-channel multiplexing.

The Cielo is designed for multiplex experiments involving up to six different targets. With the ability to scan 16 wells simultaneously, an entire 96-well plate can be scanned for all six detection channels in 9 s. It uses fibre optics to deliver light to each individual well with precision, which reduces background excitation and the need for passive reference dyes.

With the option of three or six fluorescent channels, the Cielo 3 (with three detection channels) and the Cielo 6 (six detection channels) can detect up to six different fluorophores per well and provide flexibility for experimental design. The product’s high sensitivity and resolution are designed to save the user time, money and samples, and the device comes with a 12-month standard warranty.

qPCR is used to detect specific DNA sequences in bacterial, viral and parasitic pathogens. For accurate pathogen detection, assays need to be able to detect very low amounts of genetic material. This has been especially true with SARS-CoV-2 where early detection and diagnosis are important. When tested, the company says the Cielo was able to detect as little as 0.625 copies of RNA per µL of sample, meaning it can be used for rapid detection of SARS-CoV-2.