CST SignalStar Multiplex IHC for spatial biology

Click2Contact

SignalStar Multiplex IHC, created by Cell Signaling Technology (CST), has been designed to revolutionise spatial biology research by simultaneously identifying up to eight targets in tissue samples within a short two-day timeframe. This advanced technology accelerates the multiplex immunohistochemistry (mIHC) process, with the company’s optimised, ready-to-go panels allowing users the flexibility to change targets and redesign panels quickly.

The SignalStar method is streamlined for efficiency and ease of use. It begins with the standard preparation of FFPE (formalin-fixed, paraffin-embedded) tissue sections. Then, in a significant departure from traditional methods, up to eight specially designed oligo-conjugated antibodies are applied to the tissue sections in a single step, each with the ability to target a specific protein. For visualisation, complementary oligonucleotides attached to fluorescent dyes amplify the signal of these antibodies. In experiments involving more than four targets, the process includes two rounds of imaging, each with a distinct set of fluorescent dyes, for clear and comprehensive visualisation of all targets.

This innovative approach eliminates the need for repetitive and time-consuming steps such as antibody cycling and prevents epitope loss or masking. This conserves time and enables amplified signals, even for low abundance targets, leading to sensitive results. Furthermore, SignalStar’s compatibility with standard imaging equipment integrates seamlessly into existing laboratory workflows, enhancing the efficiency and scope of spatial biology research.

Learn more at www.cellsignal.com/applications/signalstar-multiplex-ihc-overview.