Logos Biosystems QUANTOM Tx for automated single bacterial cell count

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Counting and identifying bacterial cell samples reliably can be challenging. Bacteria are an incredibly diverse group of organisms that come in multiple shapes, sizes and arrangements, making detailed assessment a difficult process. While manual cell counting of bacterial colony forming units (CFUs) remains a typical practice in laboratories, it is time-consuming, labour-intensive and provides only an estimate of the viable cells present. Even expensive flow cytometers and laser scanning cytometers still register each particle, single or clustered, as a single event. Mammalian cell counters typically are unsuitable given the small size of bacterial cells.

Logos Biosystems’ QUANTOM Tx offers a solution to these challenges with rapid bacterial quantification. QUANTOM Tx is an automated fluorescence-stained microbial cell counter with fluorescence imaging and analysis that counts bacterial cells as small as 0.3 μm, in around 30 s/sample.

Image-based analysis enables direct counting without the need for overnight culturing. Sophisticated counting algorithms allow single bacteria-cell detection regardless of genome size, morphology, cell size or motility, and even in the tightest of clusters. In addition, a special kit for counting viable bacterial cells overcomes variability. Stained cells are loaded into cell counting slides and spun in the QUANTOM Centrifuge to immobilise and evenly distribute cells to provide appropriate cell counts.

With QUANTOM Tx users can: count bacterial strains that are not culturable in lab conditions; remove user-to-user variation; analyse multiple fields of view with no need for estimation based on turbidity or CFUs; use an easy-to-follow protocol and autofocus; and assess fluorescence stains for either total count or viable count information.

For more information: https://www.atascientific.com.au/products/quantom-tx-microbial-cell-counter/.