Abraxis Glyphosate Kit

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Glyphosate is a herbicide widely used to control various types of weeds. Although it is considered to be relatively safe compared to some organochlorine pesticides, various studies have shown possible toxicological effects linked to its use (slightly toxic to invertebrate species, possibly toxic to amphibian species, increased neurological developmental effects in exposed children and loss of fertility). The Abraxis Glyphosate Kit applies the principles of enzyme linked immunosorbent assay (ELISA) to the determination of glyphosate in urine, soil, milk, lentils, white beans, soy beans, corn and malt barley.

The sample to be tested is derivatised and then added, along with an antibody specific for glyphosate to microtiter wells coated with goat anti-rabbit antibody and incubated for 30 min. The glyphosate enzyme conjugate is then added. At this point a competitive reaction occurs between the glyphosate, which may be in the sample, and the enzyme-labelled glyphosate analog for the antibody binding sites on the microtiter well. The reaction is allowed to continue for 1 h. After a washing step and addition of a substrate (colour solution), a colour signal (blue colour) is generated.

The presence of glyphosate is detected by adding the colour solution, which contains the enzyme substrate (hydrogen peroxide) and the chromogen (3,3’,5,5’-tetramethylbenzidine). The enzyme-labelled glyphosate bound to the glyphosate antibody catalyses the conversion of the substrate/chromogen mixture to a coloured product. After an incubation period, the reaction is stopped and stabilised by the addition of a diluted acid (stopping solution). Since the labelled glyphosate (conjugate) was in competition with the unlabelled glyphosate (sample) for the antibody sites, the colour developed is inversely proportional to the concentration of glyphosate in the sample.