Enzo AMPIPROBE HCV Assay Kit

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The AMPIPROBE HCV assay kit, from Enzo, is a quantitative reverse transcription polymerase chain reaction (RT-qPCR) assay for the detection of human hepatitis C virus (HCV) RNA in plasma or serum. The primer mix included in the kit is specific for HCV genotypes 1 through 6.

The kit includes titered high, medium and low HCV controls which enable virus quantitation in IU/mL. In addition, it contains an extraction control to ensure proper sample nucleic acid extraction. The product is compatible with most open qPCR platforms, with a small sample input that allows remaining extracted samples to be used in other tests.

Enzo’s AMPIPROBE technology incorporates probe detection technology in primer design. It employs a combination of fluorescent reporter-labelled primers and quencher-labelled primers to amplify DNA, akin to traditional PCR. When free in solution, fluorescent primers generate a signal. However, as the primers are incorporated into amplified DNA, the quencher and the fluorophore are brought within close proximity and exhibit Forster resonance energy transfer (FRET). This causes a logarithmic decay of signal with respect to the number of amplification cycles of DNA. Once the signal decays to a defined threshold, a value is generated with respect to the corresponding cycle. The threshold cycle is indicative of the amount of target RNA or DNA in the sample.

For more information: http://www.enzolifesciences.com/ENZ-GEN200/ampiprobe-hcv-assay-kit/.