Enzo Life Sciences Mito-ID assays for mitochondrial function

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Detecting changes in metabolism is crucial to the understanding of many pathological processes. Monitoring both oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) provides a comprehensive analysis of cellular bioenergetics. Enzo Life Sciences’ Mito-ID assays work together to assess the dynamic interaction between mitochondrial respiration and glycolytic flux.

Mito-ID Oxygen and pH Sensors are high-throughput assays with easy mix-and-measure protocols. The sensitive, reversible probes provide real-time analysis and are compatible with standard fluorescence plate readers.

Mito-ID Extracellular O2 Sensor Kits are oxygen-sensitive phosphorescent probes which can be used to assess O₂ consumption by cultured cells, isolated mitochondria, microorganisms, tissues and enzymes. The probe increases in signal intensity with O₂ consumption. It is amenable to 96- and 384-well microplates for high-throughput analysis on standard fluorescence plate readers (Ex/Em 380/650 nm). A cell-permeable probe is also available for tracing intracellular O₂ levels, and the user may multiplex with the Mito-ID pH sensor probe to confirm mitochondrial toxicity.

The Mito-ID Extracellular pH Sensor Probe is a pH-sensitive phosphorescent probe which can be used to monitor cellular acid extrusion - the result of increased glycolytic flux associated with mitotoxicity. The probe increases in signal intensity with increasing acidity. It provides a simple mix-and-read protocol for 96-well microplates and is amenable to standard fluorescence plate readers (Ex/Em 340/615 nm).

The Mito-ID Membrane Potential Cytotoxicity Assay Kit is a real-time mitochondrial membrane potential assay, said to be 10x more sensitive than JC-1 with good aqueous solubility. The product features photostable dual-emission dye and no-wash/no-medium removal. It is suitable for high-throughput applications; separate Mito-ID Red/Green assays are available for the detection of mitochondrial mass.