Enzo Life Sciences trefoil factor 1 (human) ELISA kit

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Trefoil factor 1 (TFF1) is a small secreted protein with molecular weight of 6.5 kDa (monomers, 14 kDa - dimers). It belongs to the TFF protein family that is characterised by a clover-leaf-like disulphide structure named the TFF domain, which is created by six cysteines forming three intramolecular bonds. TFF1 contains one trefoil domain, but has a seventh cysteine in position 57 that is essential for formation of dimers. TFF1 exist as both monomers and dimers. The most abundant expression of TFF1 is found in the GI tract (especially in stomach, colon and pancreas) where it is co-localised with mucins.

It is probable that TFF1 is connected with healing and stabilisation of the mucin layer. TFF1 was found in significant amounts in ulcer associated cell lineage UACL, where EGF (epidermal growth factor) is also present. The hypothesis that TFF1 expression is influenced by EGF has been proposed, and this has been supported by a study on EGF KO mice which had lower levels of TFF1. A study examining people with Crohn’s disease and inflammatory bowel disease showed that TFF1 level in serum is increased during the inflammatory state. TFF1 is also expressed in the trachea and its level increases after administration of allergen, indicating that TFF1 could be associated with asthma. Another study found that TFF1 levels are high in septic patients and that the level correlates with prognosis of the septic state. High levels of TFF1 in serum were also found in patients with prostate and other types of cancer.

Enzo Life Sciences has introduced an ELISA kit to quantify trefoil factor 1 in a wide variety of sample types - including bronchoalveolar lavage fluid, plasma or serum - for immunity and inflammation research. The ELISA is highly sensitive and can measure as little as 19 pg/mL of trefoil factor 1.