GeneCopoeia miProfile miRNA qPCR arrays

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The miProfile human miRNome miRNA qPCR arrays from GeneCopoeia are a set of nineteen 96-well plates covering 1565 of best characterised and annotated miRNAs based on miRBase V17. Each plate contains up to 84 pairs of PCR primers and 12 wells of different types of controls, which are used to monitor the efficiency of the entire experimental process - from reverse transcription to qPCR reaction.

Each miRNA-specific primer used in the qPCR arrays has been experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted miRNA. A cDNA pool, containing reverse transcript products of 10 different tissue total RNAs, was used as the qPCR validation template.

Using a universal real-time PCR condition, one can profile and analyse the miRNA expression in a high-throughput fashion. The GeneCopoeia All-in-One miRNA First-Strand cDNA Synthesis Kits and qPCR Mix Kits are suitable RT-PCR reagents for use with the arrays. The reagents are said to produce high sensitivity, efficiency and specificity.

To help distinguish single-nucleotide mismatched miRNAs, 61 single-nucleotide mismatched miRNAs from the human miRNome are further grouped together on one 96-well plate. The miProfile human single-nucleotide mismatch miRNA qPCR array is available as standalone products for users who want to study these miRNAs using specific PCR conditions.

Each miRNA primer is designed using a proprietary algorithm and experimentally validated. It is able to detect miRNA from as little as 10 pg of small RNA or 20 pg of total RNA. It is also able to distinguish miRNAs with single nucleotide mismatches. Each primer set has been experimentally validated for specific amplification.

The product allows miRNA at varieties of expression level to be detected simultaneously. It has high reproducibility (R2>0.99) for inter-array and intra-array replicates.