Abcam FirePlex-HT multiplex immunoassays

Abcam’s FirePlex-HT immunoassays offer a no-wash workflow in 384-well plate format, enabling multiplex quantification of up to 10 protein analytes per well from as little as 6.25 µL of plasma, serum or cell culture supernatant. A two-step protocol limits hands-on time and can be easily automated, making the assay suitable for high-throughput studies.

Data from FirePlex particles can be collected using any high-content imager at scan speeds of less than 20 min/plate. Subsequent analysis is performed using Abcam’s free integrated FirePlex Analysis Workbench software.

Available as pre-designed panels or user-designed custom panels, researchers can select from a growing catalogue of over 800 antibody pairs, the majority of which have been developed using Abcam’s recombinant monoclonal antibody technology. Quality antibodies combined with FirePlex particles enable consistent assays with a dynamic range of 3–4 logs, sensitivity down to 1 pg/mL and <15% intraplate CVs.