BD Influx cell sorting system

The Influx cell sorting system enables researchers to configure up to seven lasers to support two-, four- and six-way sorting, giving users flexibility to meet specific application or environmental requirements.

The system’s modular architecture along with exchangeable detector options, hands-on controls and sorting options make it adaptable to a wide variety of site and application needs. The seven laser paths and seven-pinhole optical collection system in the Influx system supports 24 parameters simultaneously with a 24 x 24 compensation matrix. Its sorting modes, such as proportional and positional sorting, have additional application flexibility.

The system uses parallel electronics to reach a throughput rate of 200,000 events/s, independent of the number of lasers or parameters. Its fluidics design has an acoustical coupling in the nozzle assembly to reliably create droplets for sorting, while ensuring low shear stress to optimise cell viability, even at high pressures.

FACS Accudrop technology simplifies set-up and eliminates manual calculations normally required for drop-delay determination. To support aseptic sorting, the Influx system’s disposable fluidics allows researchers to replace a sample line or the complete fluidics path from sheath tank to nozzle tip.

Influx software will provide control of the cell sorter from configuration and compensation set-up to acquisition, sorting and analysis. The software will use industry-standard protocols and manage hundreds of system parameters, giving researchers a higher level of control and engagement with the instrument to support advanced research applications. Software wizards and controls will assist researchers to classify cell populations, perform compensation, monitor sorting and analyse results.