Bio-Rad Laboratories StarBright Blue and StarBright Yellow Dyes

Bio-Rad Laboratories has announced its StarBright Blue and StarBright Yellow Dyes to provide flexibility in conventional and full spectrum flow cytometry applications. Designed to offer high brightness with narrow excitation and emission profiles for precise resolution, the dyes expand research capabilities across the blue (488 nm) and yellow (561 nm) lasers.

Each of the stable StarBright Dyes is conjugated to flow validated antibodies, supporting flow cytometry panel design in immunology research. Blue Dyes 580, 615, 675, 700, 765 and 810 are excitable by the 488 nm laser and Yellow Dyes 575, 605, 665, 720 and 800 by the 561 nm laser, with low background. Compared to PE and PE tandems, Blue Dyes are true 488 nm excitable dyes and Yellow Dyes offer reduced excitation by the 488 nm laser. They can be used in combination with other StarBright Dyes and traditional fluorophores.

The dyes are compatible with most experimental protocols and flow cytometers, including the Bio-Rad ZE5 Cell Analyzer, without the need for special buffers. Minimal lot-to-lot variation helps to provide reproducible and consistent staining, and the dyes are resistant to photobleaching with no loss of signal in fixation.