Bio-Rad Laboratories StarBright Violet Dyes for flow cytometry

Bio-Rad Laboratories’ line of StarBright Dyes, for flow cytometry applications, offers researchers a range of fluorescent nanoparticles conjugated to highly validated flow antibodies. The StarBright Violet 570, 670 and 710 Dyes are designed to offer precise excitation and emission spectra, making them suitable for use in multicolour flow cytometry panels.

The dyes are said to offer improved brightness when compared to other available dyes at similar wavelengths, and they may be used with a wide range of flow cytometers. The profile of the dyes offers enhanced resolution of rare populations and low-density antigens, the company says, as well as consistent and reproducible staining.

The dyes are compatible with new and existing protocols and suitable for use in multiplex panels. Resistant to photobleaching and highly stable with minimal lot-to-lot variation, they offer good Förster resonance energy transfer (FRET) for reproducible data.

The dyes work with common staining buffers, including special polymer dye staining buffers, for easy integration into multicolour panels. With no loss of signal in fixation, they are said to offer narrow excitation and emission profiles for improved resolution in new and existing multicolour flow cytometry experiments.

The dyes are compatible with Bio-Rad’s ZE5 Cell Analyzer, S3e Cell Sorter and other conventional and spectral flow cytometers.