The SuperScript Vilo cDNA synthesis kit provides reliable and sensitive first strand cDNA synthesis. The kit uses RNAse H reduced SuperScript III Reverse Transcriptase, generating greatly increased yields of cDNA and improving qPCR sensitivity.
The kit allows highly accurate linear detection in a downstream qPCR reaction from as little as 1 pg of starting RNA all the way up to 2.5 µg in a 20 µL reaction.
The kit uses an enhanced reaction mix that includes a helper protein designed for minimising qPCR inhibition, leading to improved detection.
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