CellBank Australia HeLa H2B-GFP and HeLa H2B-GFP cell lines for chromatin research
CellBank Australia has released two cell lines for the study of chromatin architecture and dynamics. The HeLa derivatives are genetically modified to carry fluorescently tagged histone H2B proteins. H2B is a core component of the nucleosome, where it plays an important role in transcriptional regulation, DNA repair and other essential cellular functions.
The first cell line, HeLa H2B-GFP, carries H2B tagged with eGFP. The second cell line, HeLa H2B-GFP, carries both a H2B tagged with eGFP and H2B tagged with mCherry. Expression of both proteins enabled study of chromatin compaction in live cells using FLIM-FRET microscopy.
The cell lines were published in PNAS in 2019, where they were used to study the remodelling of chromatin architecture at double-strand break sites during DNA repair.
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