The Cell Counting Kit-8 (CCK-8) from Dojindo is a pre-mixed, nonradioactive one-bottle solution that enables the colorimetric determination of cell viability in cell proliferation and cytotoxicity assays.
The kit utilises Dojindo’s highly water-soluble tetrazolium salt, WST-8. The WST-8 is reduced by dehydrogenase activities in cells to provide a yellow-colour formazan dye, which is soluble in the tissue culture media. The amount of formazan dye, generated by the activities of dehydrogenases in cells, is directly proportional to the number of living cells when measured in a plate reader at 450 nm.
The detection sensitivity of CCK-8 is said to be higher than the other tetrazolium salts such as MTT, XTT, MTS or WST-1; consequently, it should outperform those first-generation compounds.
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