Phosphatidylinositol phosphates (PIPs) are more than just membrane components. They are vital signalling molecules within the cell that participate in a variety of biological processes ranging from membrane trafficking to inflammation and cell death.
The production of PIP3 by class I PI3-kinases (PI3-K) is important in multiple cell signalling pathways. Typically, experiments to measure PI3-K activity have involved phosphorylation of a phosphoinositide substrate using 32P, then extraction of radioactive products and separation using thin-layer chromatography.
Developed by Echelon Biosciences, PIP Mass Assays offer researchers a comprehensive selection of ELISA-based assays for the measurement of PIP levels from biological samples. The PIP3 Mass Assay allows the user to determine PI3-K activity by measuring the amount of PIP3 extracted from cells by means of standard ELISA format, eliminating the need for radioactivity, and thin-layer chromatography.
The mass assay range is for research use only.
Phone: 02 9698 2022
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