EpiGentek EpiQuik Total Histone Extraction Kit

The EpiQuik Total Histone Extraction Kit simply applies EpiGentek’s histone isolation buffers to cell or tissue samples. After treatment with pre-lysis, lysis and balance buffers, the total core histones are easily extracted for immediate use or storage under proper conditions.

The kit offers a complete set of optimised buffers and reagents for extracting total core histone proteins (H2A, H2B, H3, and H4) from mammalian cells or tissues in a simple 60 min procedure. Offering a standardised procedure for reproducible results, it extracts a high yield of histones from as little as 1 mg of tissues. It does not affect histone modification status or levels.

The product ensures that PTMs in the histone extracts are kept intact and thus can be used in a variety of downstream applications, such as histone methylation, acetylation, phosphorylation, sumoylation, ubiquitination, citrullination and ADP-ribosylation studies.