EXBIO CellCycleFlowEx Kit for flow cytometry

Proliferating cells duplicate their DNA during the cell cycle. The DNA is subsequently segregated equally into the daughter cells. Measurement of cellular DNA content within a proliferating cellular population recognises the cells in S phase (actively duplicating their DNA), G2/M phase (having double the amount of DNA than resting cells) and G0/G1 phase (the same amount of DNA as resting cells) of the cell cycle. Analysis of the percentage of cells at different stages of the cycle according to their DNA content is used to assess the proliferative response to stimulation with mitogens (lymphocyte transformation test) or cell cycle inhibitor/s effects.

To monitor this, EXBIO offers a solution for cell cycle analysis using flow cytometry with the CellCycleFlowEx Kit. The kit is suitable for testing suspensions of isolated cells, such as leukocytes isolated from peripheral blood mononuclear cells (PBMC) or cells from tissue culture. This flow cytometric analysis method allows users to distinguish cell suspensions in G0/G1, S and G2/M phases of the cell cycle.