Innova Biosciences Lightning-Link antibody labelling kits for multicolour flow cytometry

Flow cytometry is a widely used application for cell analysis, but it is important to consider the combination of fluorescent labels for use within multicolour flow cytometry. Fluorophore characteristics, compatibility with the other fluorescent molecules and the instrument to be used for the sample analysis should all be acknowledged.

Furthermore, the availability of conjugates required for multicolour cytometry can be an issue. It is not unusual to find that the specific fluorochrome conjugate required is not available.

Lightning-Link antibody labelling kits, from Innova Biosciences, enable direct conjugation of antibodies, proteins or peptides to a fluorescent label in under 20 min for use in multicolour flow cytometry. Direct conjugation can be achieved with only 30 s of hands-on time.

The user can choose from over 35 fluorescent dyes in Lightning-Link range. If the specific antibody conjugate is not available commercially, the desired conjugate can be prepared in less than 20 min with a 100% yield from the unconjugated antibody. The simplicity and convenience of the kits provides ease and flexibility in panel design for multicolour flow cytometry.